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Journal / Developmental Biology / Middelskala forberedelse af
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Developmental Biology

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JoVE Journal Developmental Biology
Middelskala forberedelse af
Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
Journal (Developmental Biology)
Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster
Journal (Developmental Biology)
Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster
 
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Middelskala forberedelse af

Article DOI: 10.3791/55804-v • 09:16 min • May 30th, 2017
May 30th, 2017 •
1, 1, 1, 2, 1
1Biology Department, University of Massachusetts Boston, 2Department of Bioengineering, University of California, San Diego

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Formålet med denne protokol er at tilvejebringe en retfærdig og billig tilgang til indsamling af Drosophila embryoner i medium skala (0,5-1 g) og fremstilling af proteinekstrakter, der kan anvendes i nedstrøms proteomiske applikationer, såsom affinitetsrensnings-massespektrometri (AP-MS ).

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Udviklingsbiologi udgave 123 ekstrakt lysat protein, Frugt flyve embryo proteomics medium skala rensning bur
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