A Mouse 5/6<sup>th</sup> Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy

Xiaoliang Wang; Muhammad A. Chaudhry; Ying Nie; Zijian Xie; Joseph I. Shapiro; Jiang Liu

doi:10.3791/55825

Journal

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Biology

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A Mouse 5/6^th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy

A Mouse 5/6<sup>th</sup> Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy

JoVE Journal
Biology

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JoVE Journal Biology

A Mouse 5/6^th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy

20,416 Views

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07:52 min

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November 07, 2017

DOI:

10.3791/55825-v

DOI:

10.3791/55825-v

•

07:52 min

November 07, 2017

•

20361 Views

Xiaoliang Wang*¹, Muhammad A. Chaudhry*², Ying Nie², Zijian Xie¹, Joseph I. Shapiro², Jiang Liu^1,2

¹Marshall Institute for Interdisciplinary Research (MIIR),Marshall University ²Department of Biomedical Sciences,Marshall University Joan C. Edwards School of Medicine

Summary

This manuscript provides a detailed two-step surgical procedure to perform mouse 5/6^th partial nephrectomy (PNx) with pole ligation. Four weeks after surgery, in comparison with sham-operated mice, the PNx mice developed impaired renal function, anemia, cardiac hypertrophy, cardiac fibrosis, and decreased heart systolic and diastolic function.