This work details a standard immunohistochemistry method to visualize motor neuron projections of late stage-16 Drosophila melanogaster embryos. The filleted preparation of fixed embryos stained with FasII antibody provides a powerful tool to characterize the genes required for motor axon pathfinding and target recognition during neural development.
Jeong, S. Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila. J. Vis. Exp. (124), e55830, doi:10.3791/55830 (2017).