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Genetics

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In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
 

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Article doi: 10.3791/55832
September 28th, 2017

Summary September 28th, 2017

An in vitro bioluminescence assay to determine cellular circadian rhythm in mammary epithelial cells is presented. This method utilizes mammalian cell reporter plasmids expressing destabilized luciferase under the control of the PERIOD 2 gene promoter. It can be adapted to other cell types to evaluate organ-specific effects on circadian rhythm.

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