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Tuning i Hippocampal Theta Band<em> I Vitro</em>: Metoder for opptak fra isolert gnagere Septohippocampal Circuit
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit
DOI:

11:37 min

August 02, 2017

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Chapters

  • 00:05Title
  • 00:54Isolating the Hippocampus
  • 03:47Set Up Fast Perfusion for Recording the Isolated Hippocampus
  • 04:34Extracellular Recording on In Vitro Hippocampal Theta Oscillations
  • 07:57Optogenetic Control in the Isolated Hippocampus
  • 09:11Results: Cell-type Specific Activity of Pyramidal Cell and PV Interneuron During Spontaneous Theta Oscillations in the Intact Hippocampal Preparation from a PV-TOM Mouse
  • 10:19Conclusion

Summary

Automatic Translation

Her presenterer vi en protokoll for opptak av rytmisk nevronnettverk theta og gamma-svingninger fra et isolert helhippocampalpreparat. Vi beskriver de eksperimentelle trinnene fra utvinning av hippocampus til detaljer om felt-, enhetlig og helcelle patch clamp opptak samt optogenetic pacing av theta rytmen.

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