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A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays
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Chapters
- 00:00Title
- 00:30Dissection Overview
- 02:23Dissection of pupal wings
- 04:38Multiple pupae dissection
- 05:32Antibody stained pupal wings and PCR
- 06:23Conclusions
The ability to accurately detect transcripts or proteins in Drosophila tissues is critical for studying their abundance and localization related to the development process. Here is the description of a straightforward procedure to dissect pupal wings. These wings can be used as samples in several techniques (immunohistochemistry, PCR assay, etc.).
