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JoVE Journal
Developmental Biology
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A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays
 

A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays

Article DOI: 10.3791/55854 07:11 min December 30th, 2017
December 30th, 2017

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Summary

The ability to accurately detect transcripts or proteins in Drosophila tissues is critical for studying their abundance and localization related to the development process. Here is the description of a straightforward procedure to dissect pupal wings. These wings can be used as samples in several techniques (immunohistochemistry, PCR assay, etc.).

Transcript

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Rapid Method Efficient Method Dissect Pupal Wings Drosophila Immunodetections PCR Assays Wet Paintbrush Zero-hour APF Pupa Culture Vials Development Period Microscope Slide Double-sided Tape Transference Dorsal Sides Up Cephalic Ends Constant Temperature Developmental Time Pupal Case Forceps Dissection Platform
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