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Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
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JoVE Journal Neuroscience
Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

DOI:
10.3791/55865-v

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08:17 min

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September 22, 2017

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, , , , , , ,
1INSERM U1051, Institut of Neurosciences of Montpellier, 2CNRS UMS3426,BioCampus Montepellier, 3University of Montpellier, 4CHRU Montpellier, Centre of Reference for Genetic Sensory Diseases,CHU Gui de Chauliac Hospital

Chapters

  • 00:05Title
  • 00:51Pre-imaging Preparation
  • 01:51Mouse Preparation
  • 02:40Mouse Surgical Preparation
  • 04:43Spectral Domain-optical Coherence Tomography (SD-OCT) Optical Nerve Head (ONH) and Retina Imaging
  • 05:31Analysis
  • 06:25Results: Representative Retinal Scanning and Analysis
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript describes a protocol for the in vivo imaging of the mouse retina with high-resolution spectral domain optical coherence tomography (SD-OCT). It focuses on retinal ganglion cells (RGC) in the peripapillary region, with several scanning and quantifying approaches described.

Tags

Keywords: Optical Coherence TomographyOCTRetinal Ganglion CellsNon-invasiveOptic NeuropathyRetinal ThicknessMouseEyePhenylephrineTropicamideAnesthesiaOxybuprocaine HydrochlorideGlycolHypromelloseImaging

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