Journal
/
Biology
/
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy

Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy

DOI:
10.3791/55870-v

•

06:26 min

•

June 19, 2017

•

, , ,
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), 2Department of Cardiac Surgery,University of Rostock, 3Department of Life, Light and Matter of the Interdisciplinary Faculty,University of Rostock

Chapters

  • 00:05Title
  • 00:38Transfection of Cardiomyocytes with Fluorescently Labeled miRNA
  • 01:56Applying 3D-FRAP Microscopy and Data Analysis
  • 04:08Results: 3D FRAP Analysis of Gap Junction-dependent Transfer of miRNA
  • 05:42Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe the application of three-dimensional fluorescence recovery after photobleaching (3D-FRAP) for the analysis of the gap junction-dependent shuttling of miRNA. In contrast to commonly applied methods, 3D-FRAP allows for the quantification of the intercellular transfer of small RNAs in real time, with high spatio-temporal resolution.

Tags

Gap JunctionMicro RNA3D-FRAP MicroscopyCardiomyocytesCell CultureElectroporationConfocal MicroscopyFluorescence Recovery After Photobleaching (FRAP)

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

Detection of miRNA Targets in High-throughput Using the 3’LIFE Assay

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Tethered Bilayer Lipid Membranes to Monitor Heat Transfer between Gold Nanoparticles and Lipid Membranes

Application of Passive Head Motion to Generate Defined Accelerations at the Heads of Rodents

Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy

Fluorescence Lifetime Macro Imager for Biomedical Applications

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis

Read Article