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Biology

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Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
 

Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy

Article doi: 10.3791/55870
June 19th, 2017

Summary June 19th, 2017

Here, we describe the application of three-dimensional fluorescence recovery after photobleaching (3D-FRAP) for the analysis of the gap junction-dependent shuttling of miRNA. In contrast to commonly applied methods, 3D-FRAP allows for the quantification of the intercellular transfer of small RNAs in real time, with high spatio-temporal resolution.

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