Fluorescent Rna Tracking
Fluorescent Rna Tracking - Related Videos
10:16
Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
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Cited by 35 •
2012
A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.
08:39
Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy
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2025
This article provides a detailed description of how to create samples for single-protein tracking in solid-supported lipid bilayers. It also explains a straightforward fluorescence microscope with single-molecule sensitivity and a fast frame rate. Finally, we outline the procedure for extracting single-protein trajectories.
09:57
Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
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Cited by 22 •
2013
Here we describe a whole-mount fluorescent in situ hybridization (FISH) protocol for determining the expression and localization properties of RNAs expressed during embryogenesis in the fruit fly, Drosophila melanogaster.
03:49
A Luciferase-Fluorescent Reporter Influenza Virus to Track Viral Infections
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2025
This video demonstrates a method to track viral infections in mouse models using a recombinant luciferase and fluorescence protein-expressing bi-reporter Influenza A virus. This method enables both in vivo and ex vivo studies, enabling researchers to gain insights into viral dynamics and the effects of interventions.
02:59
Using Immuno-RNA Fluorescence In Situ Hybridization to Visualize SARS-CoV-2
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2025
This video demonstrates the application of Hybridization Chain Reaction (HCR)-Fluorescence In Situ Hybridization (FISH) for visualizing the spatial distribution of SARS-CoV-2 subgenomic RNA. Combining HCR with immunofluorescence techniques has the potential to provide insights into host-virus interactions at the single-cell level.
FAQs
Each labeling strategy supplies a different route to a fluorescent readout. A probe or RNA-binding protein associates with the target transcript, whereas a fluorogenic RNA aptamer produces fluorescence when activated. These approaches make the transcript visible to microscopy, enabling observation of its position, movement, abundance, or interactions in cells.
Fluorescence links the presence of a labeled transcript to an observable signal. Binding-based labels reveal the RNA when the fluorescent component associates with it, while fluorogenic aptamers reveal it through activation. Microscopy can then follow changes in these observations over time, helping researchers characterize dynamic RNA behavior rather than relying on a single measurement.
Bulk measurements provide aggregate information but do not resolve where individual RNA molecules are or how their behavior changes. Fluorescent RNA tracking adds spatial and temporal information, allowing researchers to examine localization and movement alongside abundance. This makes the method complementary to bulk analysis rather than a replacement for it.
Following labeled transcripts over time can reveal more than their presence. Researchers can examine RNA transport and localization, then observe changes associated with processing or degradation. The same time-resolved view also supports analysis of RNA interactions in cells, connecting transcript behavior with changing cellular organization.
A typical study first selects the transcript or RNA behavior to examine, then chooses a labeling strategy such as a fluorescent probe, RNA-binding protein, or fluorogenic RNA aptamer. After the label binds or becomes activated, microscopy is used to visualize the signal. Researchers can analyze location, movement, abundance, or interactions from those images.
Live-cell imaging is most valuable when the research question concerns change over time rather than a single spatial observation. It allows researchers to follow RNA transport, localization, processing, degradation, or interactions as cellular behavior unfolds. That temporal perspective can expose dynamics that a measurement taken at only one point would not show.
RNA behavior provides a direct connection between transcript-level events and gene regulation. By visualizing where RNA is located, how it moves, and how its abundance or interactions change, researchers can relate cellular organization to regulatory processes. The approach therefore adds spatial and temporal context to biological studies that otherwise rely on aggregate RNA measurements.