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Biology
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自噬研究中蛋白质-蛋白质相互作用的研究
JoVE Journal
Biology
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Biology
Study of Protein-protein Interactions in Autophagy Research
Please note that all translations are automatically generated.
Click here for the English version.
自噬研究中蛋白质-蛋白质相互作用的研究
DOI:
10.3791/55881-v
•
14:08 min
•
September 09, 2017
•
Secil Erbil-Bilir
,
Nur M. Kocaturk
,
Melih Yayli
,
Devrim Gozuacik
3
1
Molecular Biology, Genetics and Bioengineering Program
,
Sabanci University
,
2
Information Center
,
Sabanci University
,
3
EFSUN, Center of Excellence for Functional Surfaces and Interfaces for Nano Diagnostics
,
Sabanci University
Chapters
00:00
Title
01:25
Immunofluorescence
05:43
Representative Results for Immunofluorescence
06:16
Immunoprecipitation
06:38
Harvesting Cells
07:33
Protein Isolation
08:10
Bradford Assay
08:36
Antibody Coupling of Beads
09:26
Incubation of Total Cell Lysate with Beads
09:52
Elution of Proteins from Beads
10:18
Preparation of Input Controls
10:43
Denaturation of Proteins and Western Blot
13:06
Representative Results for Immunoprecipitation
13:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了两种抗体蛋白-蛋白质相互作用的研究方法: 免疫荧光和沉淀。这些技术适于研究蛋白质之间的物理相互作用, 以发现细胞信号通路的新成分, 以及了解蛋白质动力学。
Tags
Protein-protein Interactions
Autophagy
Molecular Biology
Biochemistry
Cell Biology
Immunoprecipitation
Immunofluorescence
Confocal Microscopy
Protein Localization
Cell Culture
Fixation
PFA
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