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诱导和诊断肿瘤<em>果蝇</em> Imaginal Disc Epithelia
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Cancer Research
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JoVE Journal Cancer Research
Induction and Diagnosis of Tumors in Drosophila Imaginal Disc Epithelia
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诱导和诊断肿瘤<em>果蝇</em> Imaginal Disc Epithelia

DOI:
10.3791/55901-v

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08:14 min

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July 25, 2017

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,
1Structural Biology Center, National Institute of Genetics and Department of Genetics, School of Life Science,SOKENDAI, 2Graduate School of Media and Governance,Keio University

Chapters

  • 00:05Title
  • 00:56Mosaic Clone Induction
  • 02:21Dissection of Larvae
  • 03:19Isolating and Mounting Imaginal Discs
  • 04:29Diagnosing Neoplastic Phenotypes from Confocal Image
  • 06:17Results: Tumors Induction from IgI-RNAi Expression in the Wing Disc
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

果蝇成像盘上皮的马赛克克隆分析是研究肿瘤发生的遗传和细胞机制的有力模型系统。在这里，我们描述了使用GAL4-UAS系统在果蝇翼成像盘中诱导肿瘤的方案，并介绍了分类肿瘤表型的诊断方法。

Tags

Tumor InductionDrosophilaImaginal DiscsMosaic ClonesTumor DevelopmentHeat-shockFlip-out GAL4Third-instar LarvaeDissectionFixationAntibody Staining

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