JoVE Journal
Genetics
Genetics
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Recent advances in the ability to genetically manipulate somatic cell lines hold great potential for basic and applied research. Here, we present two approaches for CRISPR/Cas9 generated knockout production and screening in mammalian cell lines, with and without the use of selectable markers.