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全自动毛细 Electrophoretic-based 免疫分析方法的程序和关键优化策略
JoVE Journal
Biology
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JoVE Journal
Biology
Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
Please note that all translations are automatically generated.
Click here for the English version.
全自动毛细 Electrophoretic-based 免疫分析方法的程序和关键优化策略
DOI:
10.3791/55911-v
•
09:32 min
•
September 10, 2017
•
Gail M Nelson
,
Jenna M Guynn
,
Brian N Chorley
1
National Health and Environmental Effects Research Laboratory
,
U.S. Environmental Protection Agency
,
2
Oak Ridge Institute for Science and Education at U.S. Environmental Protection Agency
Summary
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Automatic Translation
利用商用平台检测总蛋白制剂中靶蛋白的 capillary-based 免疫分析方法。另外, 对细胞培养模型系统进行了暴露时间、蛋白质浓度和抗体稀释度的测定参数的优化。
Tags
Capillary Electrophoresis
Immunoassay
Automated Procedure
Protein Quantitation
Cell Culture
Biofluids
Western Blot
Sample Preparation
Master Mix
Denaturing Conditions
Antibody Dilutions
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