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In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS
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JoVE Journal Chemistry
In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

DOI:
10.3791/55944-v

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09:56 min

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August 18, 2017

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, , , , , ,
1Earth and Biological Sciences Directorate,Pacific Northwest National Laboratory, 2Wiley Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory

Chapters

  • 00:05Title
  • 01:14Preparing Bubble Trap Tubing
  • 02:16Day Two: Sterilization of the SALVI Microchannel
  • 05:32Day Three: Inoculation of the SALVI Microchannel
  • 07:40Results: Chemical Profiling and Data Analysis of the Shewanella oneidensis MR1 Biofilm
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

This article presents a method for growing a biofilm for in situ time-of-flight secondary ion mass spectrometry for chemical mapping in its hydrated state, enabled by a microfluidic reactor, System for Analysis at the liquid Vacuum Interface. The Shewanella oneidensis MR-1 with green fluorescence protein was used as a model.

Tags

Keywords: In Situ CharacterizationShewanella Oneidensis MR1BiofilmsSALVIToF-SIMSEnvironmental MicrobiologyImaging Mass SpectrometrySIMSAnaerobic ChambersPTFE TubingBubble TrapSyringePEEK InjectorDrip Chamber

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