In Situ Chemical Mapping
In Situ Chemical Mapping - Related Videos
08:48
High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
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Cited by 5 •
2012
Genome assemblies based on massively parallel DNA sequencing technologies are usually highly fragmented. The development of physical chromosome maps can potentially improve genome assemblies. Here, we demonstrate innovative approaches to chromosome preparation, fluorescent in situ hybridization, and imaging that significantly increase throughput of the physical map development.
12:58
In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology
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Cited by 11 •
2015
Particle-tracking microrheology investigates the viscoelasticity of materials. Here, the technique is used to determine the viscoelasticity, creep compliance and effective crosslinking roles of different matrix components of a bacterial biofilm. The matrix consists of polymeric substances secreted by the bacteria and its components determine biofilm structure and mechanical properties.
08:00
Whole-Mount In Situ Hybridization
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2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
14:42
Remote Magnetic Actuation of Micrometric Probes for in situ 3D Mapping of Bacterial Biofilm Physical Properties
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Cited by 6 •
2014
This paper shows an original methodology based on the remote actuation of magnetic particles seeded in a bacterial biofilm and the development of dedicated magnetic tweezers to measure in situ the local mechanical properties of the complex living material built by micro-organisms at interfaces.
05:32
In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
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2023
Bis-3-chloropiperidines (B-CePs) are useful chemical probes to identify and characterize G-quadruplex structures in DNA templates in vitro. This protocol details the procedure to perform probing reactions with B-CePs and to resolve reaction products by high-resolution polyacrylamide gel electrophoresis.
FAQs
The method combines spatially resolved measurements with chemical signatures. Spectroscopic, microscopic, and mass spectrometric signals can be associated with precise positions in a sample. This pairing lets researchers interpret where particular chemical species occur, rather than examining composition without location. Spatial coordinates therefore become part of the chemical result.
Comparing chemical signals across locations reveals spatial differences in molecular composition. A reaction site may appear as a localized chemical signature, while a concentration gradient appears as a systematic change in signal across the sample. Phase boundaries can likewise be recognized where chemical characteristics change between neighboring regions. These patterns help connect local composition with chemical behavior.
Keeping species in place preserves the spatial context needed to interpret their chemistry. Measurements made without removing material can retain information about neighboring regions, interfaces, and local variations that may be altered by separating the sample. This is especially relevant when researchers want to examine chemical changes under realistic conditions rather than rely only on an isolated or rearranged sample.
An investigation first obtains measurements that vary with position, using a spatially resolved spectroscopic, microscopic, or mass spectrometric approach. Researchers then relate the measured signals to chemical signatures and organize those results according to location. The resulting map can be examined for localized species, gradients, boundaries, or composition changes. This workflow turns distributed measurements into interpretable chemical patterns.
The approach is useful when chemical composition must be interpreted alongside local structure or position. In catalysis, mapping can identify reaction sites; in materials research, it can reveal phase boundaries or compositional changes. Studies of biological interfaces and environmental samples can similarly examine chemistry within complex settings. It supports questions where location is scientifically relevant to understanding chemical behavior.
By linking chemical structure and composition to precise locations, mapping shows whether chemically different regions coincide with changes in reactivity or reaction behavior. That relationship is valuable for examining catalysis, where reaction sites are important, and for studying materials or interfaces with spatially varying chemistry. The outcome is a more locally grounded interpretation of how composition relates to chemical function.