Journal
/
Biochemistry
/
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

DOI:
10.3791/55959-v

•

09:15 min

•

November 21, 2017

•

, ,
1Department of Chemistry,McGill University, 2Department of Chemistry,York University

Chapters

  • 00:05Title
  • 00:44Sample Preparation
  • 02:11DSC Preparation
  • 05:32Collecting Thermolabile Ligand DSC Datasets
  • 07:39Results: Rapid Characterization of Biomolecular Folding and Binding Interactions with Thermolabile Ligands by DSC
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol for rapid characterization of biomolecular folding and binding interactions with thermolabile ligands using differential scanning calorimetry.

Tags

Keywords: Biomolecular FoldingBinding InteractionsDifferential Scanning Calorimetry (DSC)Thermolabile LigandThermodynamic ParametersBiocalorimetryDrug DesignLigand TitrationDialysisBuffer MatchingBiomolecule ConcentrationDegassingDSC Setup

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55959?article_type=v

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
check_url/55959?article_type=v

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
check_url/55959?article_type=v

Measuring Protein Binding to F-actin by Co-sedimentation
check_url/55959?article_type=v

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
check_url/55959?article_type=v

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
check_url/55959?article_type=v

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
check_url/55959?article_type=v

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
check_url/55959?article_type=v

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
check_url/55959?article_type=v

Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)
check_url/55959?article_type=v

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Read Article