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Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform
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1Bredesen Center for Interdisciplinary Research and Graduate Education,University of Tennessee, 2Bioscience Division and Center for Nanophase Materials Sciences,Oak Ridge national Laboratory, 3Department of Biochemistry and Cellular and Molecular Biology,University of Tennessee, 4Department of Microbiology,University of Tennessee
Chapters
- 00:05Title
- 00:46Two-layer Platform Fabrication
- 01:48Arabidopsis thaliana Seed and Device Preparation
- 03:05Planting of Arabidopsis thaliana Seeds into the Microfluidic Devices and Experimental Treatments
- 03:51Low Magnification Imaging (4-20X)
- 04:55High Magnification Imaging (63X)
- 06:11Atomic Force Microscopy
- 07:32Results: Arabidopsis thaliana Characterization in Multple Platforms
- 09:02Conclusion
This article demonstrates how to culture Arabidopsis thaliana seedlings in a two-layer microfluidic platform that confines the main root and root hairs to a single optical plane. This platform can be used for real-time optical imaging of fine root morphology as well as for high-resolution imaging by other means.
