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JoVE Journal Neuroscience
Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol
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Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

Article DOI: 10.3791/56092-v • 07:56 min • January 10th, 2018
January 10th, 2018 •
1,2,3, 3
1Chemical Biology, Chemistry Research Laboratory, University of Oxford, 2Department of Physiology, Anatomy, and Genetics, University of Oxford, 3Trans-synaptic signaling, European Neuroscience Institute

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Summary
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The AWESAM protocol described here is optimal for culturing murine astrocytes in isolation from other brain cells in a fast, simple, and inexpensive manner. AWESAM astrocytes exhibit spontaneous Ca2+ signaling, morphology, and gene expression profiles similar to astrocytes in vivo.

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Culturing In Vivo-like Murine Astrocytes AWESAM Protocol Monocultures Astrogliobiology Physical Release Uptake Morphology MRNA Expression Protein Expression Intercellular Calcium Fluctuations Dissection Area Petri Dishes Dissection Medium Embryonic Tissue Uterus Embryonic Sacs Scissors Pre-cooled Dissection Medium Skin Skull Forceps Cerebellum Cortex
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