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Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay
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JoVE Journal Behavior
Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

DOI:
10.3791/56094-v

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09:13 min

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July 13, 2017

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, , ,
1Department of Cell Research and Immunology,Tel Aviv University, 2Department of Chemistry,University of California-Davis

Chapters

  • 00:05Title
  • 00:49Preparation of Glycans and the Source Plate
  • 02:04Printing Designed Arrays
  • 02:56Processing and Developing the Arrays
  • 07:32Results: Profiles of Sialoglycan Microarrays Using Various Sialic Acid-binding Proteins
  • 08:47Conclusion

Summary

Automatic Translation

Automatic Translation

A sialoglycan microarray assay can be used to evaluate anti-Neu5Gc antibodies in human sera, making it a potential high-throughput diagnostic assay for cancer and other chronic inflammation-mediated human diseases.

Tags

Keywords: Sialoglycan MicroarrayAnti-Neu5Gc IgGGlycoimmunologyHigh-throughput ProfilingArray FabricationGlycan PrintingFluorescent DyeIgG Standard CurveNano PrinterSlide ProcessingBlockingIncubationScanning

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