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JoVE Journal
Developmental Biology

This content is Open Access.

Horizontal Whole Mount

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin

Article DOI: 10.3791/56106
August 2nd, 2017


Summary August 2nd, 2017

This work presents a novel processing and imaging protocol for thick, three-dimensional tissue cross-section analysis that enables the full exploitation of confocal imaging modalities. This protocol preserves antigenicity and represents a robust system to analyze skin histology and potentially other tissue types.


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