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立体定向激光捕获创伤性脑区显微解剖
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury
Please note that all translations are automatically generated.
Click here for the English version.
立体定向激光捕获创伤性脑区显微解剖
DOI:
10.3791/56134-v
•
09:29 min
•
September 11, 2017
•
Harris A. Weisz*
1
,
Deborah R. Boone*
1
,
Stacy L. Sell
,
Helen L. Hellmich
1
Department of Anesthesiology
,
University of Texas Medical Branch
Chapters
00:05
Title
00:59
Region Identification and Sectioning
04:01
Staining Protocol
04:50
Stereotactic Atlas Guided Laser Capture Microdissection with the LCM System
07:36
Results: LCM of Traumatic Brain Injury affected Brain Regions
08:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了利用激光捕获显微切割, 获得不同的细胞群体的样本, 从各个脑区的基因和 rna 分析。这项技术可以研究大鼠脑部特定区域创伤性脑损伤的差异效应。
Tags
Stereotactic Atlas
Laser Capture Microdissection
Traumatic Brain Injury
Frontal Cortex
Hippocampus
Molecular Analysis
Transcriptional Analysis
Cryostat
Optimal Cutting Temperature Medium
Brain Regions
Cell Types
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