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Journal / Genetics / En standardmetode til at undersøge ejendommen…
JoVE Journal
Genetics

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JoVE Journal Genetics
En standardmetode til at undersøge ejendommen mutagenicitet som en funktion af punkt Mutation reparation katalyseret af CRISPR/Cas9 og SsODN i humane celler
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En standardmetode til at undersøge ejendommen mutagenicitet som en funktion af punkt Mutation reparation katalyseret af CRISPR/Cas9 og SsODN i humane celler

Article DOI: 10.3791/56195-v • 10:08 min • August 25th, 2017
August 25th, 2017 •
1,2, 1,2
1Gene Editing Institute, Helen F. Graham Cancer Center and Research Institute, Christiana Care Health Services, 2Department of Medical Sciences, University of Delaware

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Denne protokol beskriver arbejdsgangen i et CRISPR/Cas9-baserede gen redigering system til reparation af punktmutationer i pattedyrsceller. Her bruger vi en kombinatorisk tilgang til gen redigering med en detaljeret opfølgende eksperimentelle strategi for måling indel dannelse på destinationsstedet — i det væsentlige, analysere onsite mutagenese.

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Molekylærbiologi sag 126 mutagenicitet punkt mutation reparation CRISPR/Cas9 enkeltstrenget DNA oligonukleotider gen redigering
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