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通过子代检测改变斑马鱼致死性骨骼突变显
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Developmental Biology
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JoVE Journal Developmental Biology
Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing
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通过子代检测改变斑马鱼致死性骨骼突变显

DOI:
10.3791/56200-v

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08:39 min

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September 01, 2017

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,
1Department of Craniofacial Biology,University of Colorado Anschutz Medical Campus

Chapters

  • 00:05Title
  • 01:05KASP Genotyping
  • 03:18Pairwise Cross Sibling Heterozygous and Embryo Rearing
  • 04:13Alcian Blue/Alizarin Red Cartilage and Bone Staining
  • 05:42Phenotype Scoring and Family Selection
  • 06:49Results: Selective Breeding for Skeletal Penetrance in Zebrafish
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

本议定书的目的是改变显致命的骨骼突变体表型在斑马鱼的选择性育种。致命的突变体不能成长为成年和繁殖, 因此本协议描述了通过后代测试来跟踪和选择显的方法。

Tags

Keywords: ZebrafishLethal Skeletal MutantPenetranceProgeny TestingSelective BreedingRecessive LethalKASP GenotypingGenomic DNAPCR

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