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Immunology and Infection
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Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

DOI:
10.3791/56218-v

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08:38 min

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October 30, 2017

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, ,
1Laboratory of Biochemical Genetics and Metabolism,The Rockefeller University

Chapters

  • 00:05Title
  • 00:51In Vitro Chemotaxis
  • 02:56Murine Lymphocyte Adoptive Transfer
  • 05:17Collection and Analysis
  • 06:28Results: Representative In Vitro and In Vivo Migration Analyses
  • 07:44Conclusion

Summary

Automatic Translation

Automatic Translation

Protocols for quantitative assessment of lymphocyte chemotaxis and migration are important tools for immunology research. Here, an in vitro protocol is described that permits real-time, multiplexed evaluation of cell migration, as well as a complementary in vivo technique enabling tracking of native cells to spleen.

Tags

Keywords: Monocyte ChemotaxisIn VitroIn VivoLymphocyte TraffickingImmunologyTHP-1 CellsFluorescent LabelingCell MigrationMCP-1HEPESRPMI-1640C57BL/6 MouseAdoptive Transfer

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