Adoptive Transfer
Adoptive Transfer - Related Videos
08:06
Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model
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Cited by 21 •
2010
An experimental lung metastasis and CTL immunotherapy mouse model for analysis of tumor cells-T cell interaction in vivo.
03:16
A Method for the High Salt Treatment of Dendritic Cells and their Adoptive Transfer into Mice
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2025
This video demonstrates a procedure for the adoptive transfer of high salt-treated antigen-presenting dendritic cells (DCs) into mice. Murine CD11c+ dendritic cells (DCs) are exposed to a medium containing high amounts of sodium, forming isolevuglandin (IsoLG)-protein adducts in the cells. These adducts serve as neoantigens and are presented on the surface of DCs. The high salt-treated cells are injected into mice through adoptive cell transfer.
07:37
An Adoptive Transfer Model of Rheumatoid Arthritis in Mice
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2025
Here, we report a protocol establishing a rheumatoid arthritis (RA) mouse model through adoptive transfer of CD4+ T cells from SKG mice, providing a rapid and reliable experimental tool for investigating the immunological mechanisms, pathological progression, and development of new treatments for RA.
06:27
Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
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Cited by 21 •
2013
We provide a reproducible method to induce type 1 diabetes (T1D) in mice within two weeks by the adoptive transfer of islet antigen-specific, primary CD4+ T cells.
13:34
Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors
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2014
This protocol describes experimental procedures to assess the differentiation of plasmacytoid dendritic cells in Peyer’s patch from common dendritic cell progenitors, using techniques involving FACS-mediated cell isolation, hydrodynamic gene transfer, and flow analysis of immune subsets in Peyer’s patch.
FAQs
Their activity depends on interactions with the recipient’s biological environment. Transferred cells may recognize antigens, release signaling molecules, or directly eliminate target cells. These distinct actions allow researchers to examine how immune cells influence host responses, pathogen-related processes, tumor biology, or other physiological outcomes after they enter the recipient.
Ex vivo preparation allows researchers to select specific immune-cell populations and alter their state before administration. Expansion can increase the available cellular material, while modification can change how cells function or respond. Comparing prepared cells with untreated populations helps link their characteristics to observed immune or physiological effects in the recipient.
The transferred material determines which cellular properties enter the recipient. Donor-derived cells provide a biological population for testing, whereas laboratory cultures offer a controlled source that can be isolated, expanded, or modified before use. This choice affects the questions an experiment can address, including cell function, immune responses, and interactions with disease-related targets.
The method separates the contribution of introduced cells from processes already present in the recipient. Researchers can prepare a defined immune-cell population, place it into a different biological setting, and then examine resulting antigen recognition, signaling, or target-cell elimination. This makes the approach useful for studying how cellular activity changes across host contexts.
A typical workflow begins by obtaining cells from a donor or laboratory culture and selecting the population relevant to the study. Researchers may then expand or modify the cells ex vivo before introducing them into a recipient. Subsequent observations focus on physiological or immune effects, such as signaling, antigen recognition, or elimination of target cells.
Researchers apply the method when they need to investigate the behavior or consequences of living cellular material in a recipient. In biology, supported uses include studying immune-cell function, host–pathogen interactions, transplantation, and tumor biology. The approach can also test whether prepared cells produce a desired physiological or immune effect.
It can show whether introduced immune cells recognize relevant antigens, release signaling molecules, or directly eliminate target cells. These observations provide functional evidence about interactions between immune cells and tumor-associated targets, while also helping researchers examine broader immune responses. Such findings support investigation of how cellular activity may contribute to disease-related outcomes.
Clinical adoptive cell therapy applies the same general strategy of preparing cellular material for introduction into a recipient, with the aim of producing a beneficial effect. The overview identifies cancer and other diseases as relevant applications. In research, related experiments also clarify cell function and mechanisms that may inform the therapeutic potential of these approaches.