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Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo
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Developmental Biology
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JoVE Journal Developmental Biology
Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo

Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo

DOI:
10.3791/56238-v

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13:21 min

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September 30, 2017

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, , , , , ,
1Laboratory of Neurosciences and Circadian Rhythms, School of Medicine,Universidad de los Andes, 2Biophysics Group, Department of Physics,Universidad de los Andes, 3Laboratory of Basic Medical Sciences, School of Medicine,Universidad de los Andes, 4Department of Microbiology and Immunology,University of North Carolina, 5Notre Dame Initiative for Global Development,University of Notre Dame, 6USAID Research and Innovation Fellowship program

Chapters

  • 00:00Title
  • 00:13Introduction
  • 03:09Obtaining Embryos for Injection
  • 04:13Preparing Injection Material
  • 05:38Obtaining Labeled T. cruzi Parasites for Injection
  • 07:04Injecting Zebrafish Larvae
  • 08:10LSFM Mounting and Imaging of Injected Larvae
  • 08:57LSFM Imaging of Injected Larvae
  • 10:44Representative Results
  • 11:40Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol, fluorescently labeled T. cruzi were injected into transparent zebrafish larvae, and parasite motility was observed in vivo using light sheet fluorescence microscopy.

Tags

Keywords: Trypanosoma CruziChagas DiseaseZebrafish LarvaeIn Vivo ModelParasite MotilityLight Sheet Fluorescence MicroscopyTransparent Casper LineHost-pathogen Interactions

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