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Neuroscience

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Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
 

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Article DOI: 10.3791/56244
December 4th, 2017

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Summary December 4th, 2017

A method for mass spectrometric analysis of endogenous peptides in human cerebrospinal fluid (CSF) is presented. By employing molecular weight cut-off filtration, chromatographic pre-fractionation, mass spectrometric analysis and a subsequent combination of peptide identification strategies, it was possible to expand the known CSF peptidome nearly ten-fold compared to previous studies.

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