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CRISPR/Cas9 蛋白注射到沟鲶、鮰松毛虫、基因编辑的胚胎
JoVE Journal
Genetics
Author Produced
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JoVE Journal Genetics
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
DOI:

16:08 min

January 20, 2018

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Chapters

  • 00:00Title
  • 01:14Brood Stock Selection and Spawning
  • 04:16Sperm Preparation
  • 05:33Egg Collection and Fertilization
  • 08:07Needle Pulling and Loading
  • 10:20Microinjection of Catfish Embryos
  • 12:34Results
  • 15:24Conclusion

Summary

Automatic Translation

本文介绍了一种简单高效的 CRISPR/Cas9 系统在沟鲶胚胎基因编辑中的微注射协议。在这个协议中, 引导 rna 和 Cas9 蛋白被微量到一细胞胚胎的卵黄中。通过敲出两条沟鲶免疫相关基因来验证这个协议。

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