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激光内在人肺组织中的新型细菌特异探针的光学筛选
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy
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激光内在人肺组织中的新型细菌特异探针的光学筛选

DOI:
10.3791/56284-v

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09:24 min

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November 29, 2017

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, , , ,
1EPSRC Proteus Hub, MRC Centre of Inflammation Research, Queen’s Medical Research Institute,University of Edinburgh, 2School of Chemistry, EaStChem,University of Edinburgh

Chapters

  • 00:05Title
  • 00:44Preparation of Ex Vivo Human Lung Tissue
  • 01:53Setup of the Confocal Laser Endomicroscopy (CLE) Device
  • 03:55Data Collection with CLE
  • 05:59Data Analysis
  • 07:41Results: FCFM Imaging of Labeling Bacteria with Human Lung Tissue
  • 08:28Conclusion

Summary

Automatic Translation

Automatic Translation

这项技术描述了一个有效的筛选过程, 以评估细菌特异性光学成像剂在前体内人肺组织, 通过纤维共聚焦荧光显微镜快速鉴定小分子化学具有可翻译潜力的候选者。

Tags

Optical ImagingConfocal Laser EndomicroscopyBacteria-specific ProbesMolecular ImagingImaging ProbesCalcein AM-labeled BacteriaFibered Confocal Fluorescence MicroscopyLaser Scanning UnitFCFM Imaging Fiber Calibration

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