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1Centre for Developmental Neurobiology,King’s College London, 2Interdisciplinary Bioengineering Graduate Program,Georgia Institute of Technology, 3Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology, 4School of Chemical & Biomolecular Engineering,Georgia Institute of Technology
Chapters
- 00:05Title
- 00:54Preparation of Bacterial Cultures and Dilution for Experiments
- 03:05Treatment of Reporter Strains with egg-5 RNAi
- 04:55Initiation of Broad-range DR
- 07:19Results: Information in C. elegans Quantified During Lifespan Modulation Through Broad-range Dietary Restriction
- 08:44Conclusion
Here, we present a framework to relate broad-range dietary restriction to gene expression and lifespan. We describe protocols for broad-range dietary restriction and for quantitative imaging of gene expression under this paradigm. We further outline computational analyses to reveal underlying information processing features of the genetic circuits involved in food-sensing.
