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Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice
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Neuroscience
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JoVE Journal Neuroscience
Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

DOI:
10.3791/56297-v

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07:04 min

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January 07, 2019

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, , , , ,
1Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Department of Anatomy and Cell Biology,Indiana University School of Medicine, 2Department of Spinal Cord Injury and Repair, Trauma and Orthopedics Institute of Chinese PLA,General Hospital of Jinan Military Region, 3Department of Orthopedics, Shandong Cancer Hospital,Shandong University, 4Department of Neurobiology, Institute of Basic Medical Sciences,Academy Military Medical Sciences

Chapters

  • 00:04Title
  • 00:53Chronic Cranial Window Preparation
  • 04:11Two-photon Imaging
  • 04:55Results: Representative Calcium and Dendritic Imaging
  • 05:53Conclusion

Summary

Automatic Translation

Automatic Translation

We describe an experimental procedure for measuring neuronal activity through dual optical windows above bilateral primary somatosensory corticies (S1) in Thy1-GCaMP6s transgenic mice using 2-photon (2P) microscopy in vivo.

Tags

Keywords: Two-photon ImagingThy1-GCaMP6s Transgenic MicePrimary Somatosensory CortexNeural Activity RecordingIn Vivo ImagingCranial Window Surgery

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