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Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae
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1Department of Biomedical Engineering,University of Wisconsin-Madison, 2Morgridge Institute for Research,University of Wisconsin-Madison, 3Laboratory for Optical and Computational Instrumentation,University of Wisconsin-Madison, 4Cellular and Molecular Pathology Graduate Program,University of Wisconsin-Madison, 5Department of Medical Microbiology and Immunology,University of Wisconsin-Madison, 6Department of Pediatrics,University of Wisconsin-Madison
Chapters
- 00:05Title
- 00:57Design and 3D Printing of Molds
- 02:57PDMS Fabrication of zWEDGI Device
- 04:43Plasma-bonding zWEDGI to Glass Dish
- 07:19Channel Preparation and Loading Larvae
- 09:52Wounding and Imaging Larvae and End of Experiment
- 11:14Results: Post Wound Live Imaging and 3D Reconstruction of Zebrafish Larvae Tail Fin
- 12:36Conclusion
This manuscript describes the zWEDGI (zebrafish Wounding and Entrapment Device for Growth and Imaging), which is a compartmentalized device designed to orient and restrain zebrafish larvae. The design permits tail transection and long-term collection of high-resolution fluorescent microscopy images of wound healing and regeneration.
