Journal
/
/
利用下一代测序检测 CtDNA 中的稀有突变
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
DOI:

11:11 min

August 24, 2017

, , , , , , , , ,

Chapters

  • 00:05Title
  • 01:04DNA Extraction from Peripheral Blood for cfDNA and Genomics DNA (gDNA)
  • 03:02Library Preparation
  • 06:47Targeted DNA Capture
  • 09:10Results: Next Generation Sequencing to Detect Rare Mutations in ctDNA
  • 10:12Conclusion

Summary

Automatic Translation

这篇手稿描述了一种检测低频突变的技术, ctDNA, ER Seq。该方法以其独特的双向误差校正、特殊的背景滤波器和有效的分子获取方式来区分。

Related Videos

Read Article