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Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
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JoVE Journal Neuroscience
Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

DOI:
10.3791/56358-v

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10:24 min

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December 03, 2017

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,
1Neuroscience Graduate Group, Perelman School of Medicine,University of Pennsylvania, 2Department of Psychiatry, Children’s Hospital of Philadelphia,University of Pennsylvania School of Medicine

Chapters

  • 00:05Title
  • 00:44Culturing mESCs
  • 02:14Differentiation mESCs Toward MGE-like Telencephalic Progenitors
  • 04:57Somatostatin-enriching Protocol: “DD12 GFP High Sonic Hedgehog (SHH)”
  • 06:36PV-enriching Protocol: “DD11/DD17 mCherry + aPKCi”
  • 07:53Generation of Nkx2.1::mCherry and Lhx6::GFP Interneuron Precursors
  • 09:11Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method for generating cortical interneuron progenitors and post-mitotic interneuron precursors from mouse embryonic stem cells using a modified embryoid body-to-monolayer method. These progenitors/precursors can be used in vitro or fluorescently sorted and transplanted into neonatal neocortex for studying fate determination, or used in therapeutic applications.

Tags

Keywords: Mouse Embryonic Stem CellsCortical Interneuron ProgenitorsNkx2.1ParvalbuminSomatostatinMEF Feeder CellsTrypsin-EDTAGelatin-coated PlatesKSR-N2 MediaEmbryoid Bodies

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