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Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
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Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Article DOI: 10.3791/56358-v • 10:24 min • December 3rd, 2017
December 3rd, 2017 •
1,2, 1
1Neuroscience Graduate Group, Perelman School of Medicine, University of Pennsylvania, 2Department of Psychiatry, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine

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Summary
Automatic Translation

This protocol describes a method for generating cortical interneuron progenitors and post-mitotic interneuron precursors from mouse embryonic stem cells using a modified embryoid body-to-monolayer method. These progenitors/precursors can be used in vitro or fluorescently sorted and transplanted into neonatal neocortex for studying fate determination, or used in therapeutic applications.

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Mouse Embryonic Stem Cells Cortical Interneuron Precursors Modified Embryoid Body Method Monolayer Method Nkx2.1 Expressing Interneuron Progenitors Parvalbumin Interneurons Somatostatin Interneurons MEF Feeder Cells MEF Media MESC Media Gelatin-coated Plates
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