/
/
Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:10Surgical Procedure for FITC-albumin Infusion into the Jugular Vein
- 03:57Histological Sectioning of Brain Infusion with FITC-albumin
- 04:47Fluorescence Microscopy and Acquisition of Color Images
- 05:44Results: FITC-albumin Extravasation as a Marker of Blood-brain Barrier Damage
- 06:44Conclusion
In this study, we present an easy and efficient procedure for evaluating the disruption of the blood-brain barrier under neurodegenerative conditions. To achieve our objective, we infused high molecular weight fluorescein isothiocyanate labelled (FITC)-albumin into mouse jugular vein and evaluated its leakage into the brain parenchyma by fluorescence microscopy.
