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Biochemistry

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JoVE Journal Biochemistry
Eine Alternative Kultur-Methode, um genomische Hypomethylierung von embryonalen Stammzellen der Maus mit MEK-Inhibitor PD0325901 und Vitamin C aufrechtzuerhalten
Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Journal (Biochemistry)
Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
Journal (Biochemistry)
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
 
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Eine Alternative Kultur-Methode, um genomische Hypomethylierung von embryonalen Stammzellen der Maus mit MEK-Inhibitor PD0325901 und Vitamin C aufrechtzuerhalten

Article DOI: 10.3791/56391
June 1st, 2018 •
1, 1, 1
1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences

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June 1st, 2018

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Wir sind zwei Chemie-basierte Protokolle für die Kultivierung embryonalen Stammzellen der Maus ausführlich beschrieben. Diese neue Methode nutzt synergistische Mechanismen der Förderung Tet-vermittelten Oxidation (durch Vitamin C) und de-Novo -Synthese von 5-Methylcytosine (durch PD0325901) zu unterdrücken, um DNA-Hypomethylierung und Pluripotenz von embryonalen Stammzellen der Maus zu erhalten.

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