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Journal / Cancer Research / Target Cell pre berigelse og hele genom…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Target Cell pre berigelse og hele genom forstærkning for enkelt celle Downstream karakterisering
Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay
Journal (Cancer Research)
Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay
Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues
Journal (Cancer Research)
Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues
 
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Target Cell pre berigelse og hele genom forstærkning for enkelt celle Downstream karakterisering

Article DOI: 10.3791/56394-v • 10:12 min • May 15th, 2018
May 15th, 2018 •
*1, *1, 1, 2, 3, 3, 4, 1,5, 1
1Institute of Cell Biology, Histology and Embryology, Medical University of Graz, 2Institute of Pathology, Medical University of Graz, 3Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, 4Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, 5Sahlgrenska Cancer Center, University of Gothenburg
* These authors contributed equally

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Denne protokol er at restituere sig og forberede Molekylær genetisk karakterisering på niveauet encellede sjældne målceller fra en blanding med ikke-målarter baggrund celler. DNA kvalitet er lig med ubehandlede enkelt celler og giver mulighed for enkelt-celle ansøgning (begge screening baseret og målrettet analyse).

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Kræftforskning spørgsmålet 135 sjældne celle analyse enkelt celle analyse samlede genom forstærkning array sammenlignende genom hybridisering i vivo afspærringsanordning målrettet næste generation sequencing næste generation sequencing immunfluorescens mærkning
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