Cytocentrifugation
Cytocentrifugation - Related Videos
02:30
Single Cell Sampling Using Laser Capture Microdissection: A Technique to Harvest Target Cells from a Heterogeneous Cell Population
0 Views •
2023
This video describes the technique for obtaining a single cell from a heterogeneous cell population using laser capture microdissection. The single isolated cell can be used for further genomic studies or cancer research.
04:05
CTC Immunofluorescence Assay : A Technique to Characterize Circulating Tumor Cells
0 Views •
2023
This video describes the Circulating Tumor Cells (CTC) Immunofluorescence assay, a technique to characterize the biomarkers on circulating tumor cells in non-small cell lung cancer patient samples. This assay could be used to monitor dynamic changes of the tumor and immune response, which may reflect the response to chemotherapy, radiation, and possible immunotherapy treatments.
03:12
May-Grunwald Giemsa Staining: A Method to Stain Bone Marrow Cells
0 Views •
2023
This video describes the technique of staining bone marrow cells using May- Grünwald Giemsa Staining. In the protocol, we will stain the murine myeloid leukemia cells, C149 cells, to analyze the morphology and differential counting.
08:09
Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection
0 Views •
Cited by 11 •
2017
We explored a tubal cytologic method by sampling the fallopian tube directly post-surgical excision as a tool of ovarian cancer early detection. Here, we present a protocol to collect fallopian tube cells from freshly received surgical specimens.
08:00
A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
0 Views •
Cited by 29 •
2016
This manuscript provides a technical procedure that can be used to characterize C1498 cell cultures in vitro and the acute leukemia induced in mice after their injection. Phenotypic and functional analyses are performed using flow cytometry, immunofluorescence microscopy, cytochemistry and May-Grünwald Giemsa staining.
FAQs
Controlled centrifugal force directs suspended cells toward the slide while the specialized chamber manages the surrounding liquid. This concentrates dispersed material into a defined spot rather than leaving cells distributed throughout the original sample volume. The localized deposit gives investigators a practical area for staining and microscopic examination, which is especially useful when cells are sparse.
Preserved cellular morphology allows investigators to examine cells microscopically after concentration, rather than relying only on their presence in the original suspension. Structural features can support the detection and characterization of malignant cells, while the prepared area can also be used for staining, immunocytochemistry, or related analyses. This connects sample preparation directly with cytological interpretation.
The technique gathers dispersed cells into a small, defined area on the slide, making the available cellular material more accessible for examination. This is valuable when sample volume or cell number is limited because investigators can evaluate a concentrated deposit instead of searching across a less concentrated preparation. In cancer research, that can support analysis of rare cellular populations.
A liquid cell suspension is loaded into a specialized chamber positioned with a microscope slide. Controlled centrifugation then drives the cells toward the slide, while excess fluid is removed or absorbed. The resulting cell spot can be prepared for staining and examined microscopically. This sequence converts a dilute suspension into a slide-based preparation suitable for cytological analysis.
Cancer researchers can apply the approach to cells recovered from body fluids, cultured samples, and other cell suspensions. These sample types may contain dispersed or uncommon cells that benefit from concentration before examination. Preparing them on a slide supports subsequent microscopy, staining, immunocytochemistry, or related analyses while retaining cellular morphology for interpretation.
A prepared slide can support cytological assessment of cell morphology and the detection or characterization of malignant cells. Staining and microscopic examination provide the main routes for evaluating the concentrated material, while immunocytochemistry or related analyses can extend the assessment. The method is therefore useful when researchers need to examine limited cellular material in a structured slide preparation.