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Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
This content is Free Access.
1Institute of Cell Biology, Histology and Embryology,Medical University of Graz, 2Institute of Pathology,Medical University of Graz, 3Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, 4Department of Tumor Biology,University Medical Center Hamburg-Eppendorf, 5Sahlgrenska Cancer Center,University of Gothenburg
Chapters
- 00:05Title
- 01:28Preparation of Target Cells
- 02:41Charging the Wire
- 03:27Counting Cells on the Wire
- 04:03Detachment and Recovery of Target Cells
- 05:25Single-cell Sampling Using Micromanipulation
- 06:37Single-cell Sampling Using Laser Microdissection
- 07:47Results: Use of Functionalized Wire for Ex Vivo Characterization and Genomic Analysis at Single Cell Level
- 08:59Conclusion
This protocol is to recover and prepare rare target cells from a mixture with non-target background cells for molecular genetic characterization at the single-cell level. DNA quality is equal to non-treated single cells and allows for single-cell application (both screening based and targeted analysis).
