Name: Autologous Microfractured and Purified Adipose Tissue for Arthroscopic Management of Osteochondral Lesions of the Talus (Video) | JoVE Journal
Uploaded: 2018-01-23T13:00:00.000+00:00
Duration: 7 min 24 s
Description: Learn arthroscopic treatment of osteochondral talar lesions using autologous microfractured adipose-derived stem cells. This technique enables cartilage regeneration with minimal soft tissue dissection and faster patient recovery.

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5 minutes

The procedures are performed using the Lipogems System.

All procedures performed in the studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee, and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
1. Medical History
<ol>
	<li>Start clinical examination with a detailed patient history. 
	NOTE: An OLT must always be suspected in case of instability of the ankle with repeated sprains associated with swelling, stiffness, pain, and joint blockage. Moreover, in many cases OLT may be associated with a history of systemic disease, such as inflammatory or vascular disease, neuropathy or neurologic disease, and diabetes. The use of drugs or medical issues that may influence healing must be evaluated and taken into account.</li>
</ol>
2. Clinical Examination
<ol>
	<li>Evaluate the patient in an orthostatic position to highlight ankle or hind-foot deformity. Assess muscle and tendon function and ankle range of movement (ROM). A diffuse tenderness is often present, especially during maximum flexion and extension, and it is not uncommon to encounter a touch-sensitive area at the level of the articular joint.</li>
	<li>Perform an anterior and posterior drawer test to identify a concomitant lateral ankle instability.</li>
	<li>During the preoperative consultation, record the following clinical and functional scores: American Orthopedic Foot &#38; Ankle Society (AOFAS) ankle and hind-foot scores15, Visual analogue scale (VAS) pain score16, and 12-Item Short Form Health Survey (SF-12)17.</li>
</ol>
3. Radiological Assessment
<ol>
	<li>Perform a bilateral weight bearing radiograph of the foot and ankle. This consists of conventional weight-bearing anteroposterior (AP) mortise, and lateral weight-bearing views of the ankle. Perform AP mortise in neutral position, and with 15 degrees of internal rotation for a better visualization of the talus. 
	NOTE: Only 50% of OLT can be diagnosed with radiograph; in case of big lesions, an area of detached bone surrounded by radiolucency can be noted18.</li>
	<li>Perform a conventional computed tomography-scan (CT-scan) of the ankle. A CT-scan allows a precise location and size of the lesion, identifying also bone fragments in case of detachment. The weak point of the CT is the ability to show the status of the cartilage. A previous study showed a sensitivity and specificity of 0.81 and 0.99, respectively, for detecting OLTs on CT19,20.</li>
	<li>Perform magnetic resonance imaging (MRI) of the ankle. MRI is fundamental in cartilage and subchondral bone assessment. Moreover, the MRI does not involve ionizing radiation, and allows a better visualization of soft-tissue. The literature reports a sensitivity and specificity of 96% in detecting OLTs21,22.</li>
</ol>
4. Surgical Technique
<ol>
	<li>Harvesting and processing of the adipose tissue
	<ol>
		<li>Prepare Klein solution: 1 L of 9 g/L saline, 50 mL of 1% lidocaine, 1 mL of 1:1000 epinephrine, 10 mL of 8.4% sodium bicarbonate, and 0.1 mL of 10 mg/mL of triamcinolone acetonide.</li>
		<li>Create two para-umbilical incisions of about 0.5 cm with a scalpel blade. Inject approximately 300 mL of Klein solution into the subcutaneous fat tissue of the abdomen through the incisions using 60 mL syringes with a 18G needle (Figure 1).</li>
		<li>Harvest 40 - 45 mL of ADSCs using a 13G blunt cannula attached to a 20-mL syringe and introduced into the processing kit (Figure 2). Typically, perform the harvesting in the peri-umbilical area.</li>
		<li>Insert 100 - 130 mL of lipoaspirate in the closed system. Push the lipoaspirate into the device through a large filter to obtain a first cluster reduction; at the same time, a corresponding quantity of saline solution exits toward the wasting bag. A key role is played by stainless steel marbles to obtain a temporary emulsion between oil, blood, and saline. Remove oil residues and contaminated blood by a gravity counter-flow of saline solution.</li>
		<li>After this washing step (the flowing solution appears clear and the lipoaspirate yellow), stop the saline flux and reverse the device (gray cap up), leading to the second adipose cluster reduction. Obtain the reduction by pushing the floating adipose clusters through the second cutting hexagonal filter, pushing fluid from below with a 10-mL syringe. Collect the final product into a 10-mL syringe connected to the upper opening of the device. 
		NOTE: The processing kit for fat tissue improves standard lipofilling technique: in fact, the system consists of a closed, full-immersion, low-pressure cylindrical system, in order to obtain a fluid and uniform product containing a great number of pericytes. This procedure allows the processing of adipose cells exclusively via mild mechanical forces, and preserving the integrity of the stromal vascular niche. The process is the least traumatic possible, and makes the final product available in a short time (15 - 20 min), without enzymatic treatments or expansion. Undamaged vasculostromal niches help the healing process.</li>
		<li>Once the ADSCs have been harvested, apply a compression bandage on the abdomen.</li>
	</ol>
	</li>
	<li>Surgical procedure and Adipose Tissue Injection
	<ol>
		<li>Place the patient in a supine position under spinal anesthesia with a tourniquet, at a pressure of 250 mmHg, at the level of the thigh to decrease the bleeding and allow a better arthroscopic visualization1.</li>
		<li>Mark the anatomical landmarks on the skin with a dermographic pen. Landmarks are essential to avoid iatrogenic injuries. 
		Mark the following (Figure 3): 
		both malleoli (lateral (LM) and medial (MM)) 
		the anterior joint line (JL), identified with dorsi- and plantar-flexion of the ankle joint 
		the tibialis anterior tendon (TAT), and Achilles tendon 
		the great saphenous vein, which runs just ahead of the medial malleolus 
		the superficial peroneal nerve (SPN)</li>
		<li>First, perform the anteromedial portal just medial to the tibialis anterior tendon, coinciding with a soft spot. This portal represents the approach portal. In most cases, a depression with the ankle in dorsiflexion is visible and palpable.
		<ol>
			<li>Cut only the skin with a blade, and then perforate the capsule by blunt dissection. Take care to avoid the saphenous nerve and the great saphenous vein. The saphenous vein is located 9 mm laterally to the portal, while the nerve is about 7.4 mm lateral to the portal. This constitutes one of two primary viewing portals.</li>
		</ol>
		</li>
		<li>Check the joint line, place the anterolateral portal, medial to the lateral malleolus, and lateral to the extensor digitorum tendon. 
		NOTE: When performing the anterolateral portal, prevent any injuries to the intermediate dorsal cutaneous nerve (the lateral branch of the superficial peroneal nerve); for this reason, after cutting, the skin must be followed by blunt dissection.</li>
		<li>Inspect the articular cartilage to assess size and position. Evaluate the condition and the quality of the cartilage with the palpator. Osteochondral lesions of the talus are usually located either posteromedially or anterolaterally.
		<ol>
			<li>Perform arthroscopic treatment using wide-angle 2.7-mm arthroscopes with a 30&#176; viewing angle, though some surgeons will use a larger 4 mm arthroscope, and keep the instrument in the anterior recess of the joint. Non-invasive joint distraction techniques and hyper plantar flexion can be used to access most of the talar dome.</li>
			<li>In case of a posterior lesion, percutaneously place a Hintermann spreader to distract the joint and allow exposure of the lesion. The Hintermann spreader has an opening lever arm applied on two K-wires previously positioned in the tibia and talar bone medially or laterally, according to the lesion side. In the case of a lateral lesion, take care to insert the proximal K-wire in the tibial bone, avoiding the fibula, to achieve better distraction of the joint.</li>
		</ol>
		</li>
		<li>Prepare the lesion with a curette, removing the damaged and unstable cartilage, the calcified layer and necrotic and sclerotic bone in order to create a regular-shaped contained lesion with shouldered borders. For this step, use a standard arthroscopic curette (Figure 4).</li>
		<li>Stimulate bone marrow stem cells from subchondral bone performing microperforations roundly from the outside to the inside of the lesion.
		<ol>
			<li>Carry out perforations with intervals of about 3 mm between them. Induce microfractures using a Chondral Pick on the healthy subchondral bone underneath the defect (Figure 5). 
			NOTE: A hemorrhage results in the formation of a fibrin clot. Blood clotting products, via inflammatory cascade activation, release vasoactive mediators, growth factors, and cytokines. These factors have the power to stimulate vascular invasion and migration of mesenchymal stem cells into the chondral portion of the lesion. These pluripotent cells are stimulated to differentiate into fibroblasts, chondrocytes, and osteoblasts, and play an important role in the stimulation of repair of the lesion. Paracrine growth factors in the articular environment promote the formation of extracellular matrix and the production of fibrocartilage. At the same time, cells of the bone part of the lesion produce an immature bone tissue that is progressively replaced by a mature bone.</li>
		</ol>
		</li>
		<li>Remove the intra-articular water using the shaver in aspiration and the remaining liquid using a cotton sponge until the joint is completely dry.</li>
	</ol>
	</li>
	<li>Adipose-derived stem cell injection
	<ol>
		<li>Inject 5 - 7 mL of adipose-derived stem cells, previously prepared in Step 4.1.3, into the ankle joint using one of the two portals (anteromedial or anterolateral).</li>
		<li>Release the tourniquet.</li>
	</ol>
	</li>
</ol>
5. Postoperative Care
<ol>
	<li>Avoid ankle movements for 15 days, with the operated limb in full discharge.</li>
	<li>15 days after surgery, allow active and passive movements of the ankle, until full recovery and range of motion is reached.</li>
	<li>Allow charge 30 days after surgery.</li>
</ol>
6. Clinical and Radiographic Follow-Up
<ol>
	<li>Evaluate patients clinically and radiologically at six and twelve months and, thereafter, annually. The follow-up protocol consists of AOFAS ankle and hind-foot scores, VAS pain score, and SF-12 at each end-point15,16,17. Radiographic examination includes an MRI and CT-scan of the operated ankle.</li>
</ol>

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<li>Usuelli, F. G., de Girolamo, L., Grassi, M., D'Ambrosi, R., Montrasio, U. A., Boga, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((All-Arthroscopic+Autologous+Matrix-Induced+Chondrogenesis+for+the+Treatment+of+Osteochondral+Lesions+of+the+Talus%5BTitle%5D)+AND+%22Arthrosc+Tech%22%5BJournal%5D)">All-Arthroscopic Autologous Matrix-Induced Chondrogenesis for the Treatment of Osteochondral Lesions of the Talus.</a> Arthrosc Tech. 4 (3), e255-e259 (2015).</li>
<li>Simonson, D. C., Roukis, T. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Safety+of+ankle+arthroscopy+for+the+treatment+of+anterolateral+soft-tissue+impingement%5BTitle%5D)+AND+%22Arthroscopy%22%5BJournal%5D)">Safety of ankle arthroscopy for the treatment of anterolateral soft-tissue impingement.</a> Arthroscopy. 30 (2), 256-259 (2014).</li>
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<li>Looze, C. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evaluation+and+Management+of+Osteochondral+Lesions+of+the+Talus%5BTitle%5D)+AND+%22Cartilage%22%5BJournal%5D)">Evaluation and Management of Osteochondral Lesions of the Talus.</a> Cartilage. 8 (1), 19-30 (2017).</li>
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Federico Giuseppe Usuelli, MD, reports personal fees from Integra and Geistlich, and grants and personal fees from Zimmer, outside the submitted work.

In recent years, clinical and preclinical trials have focused their attention on the effect of ADSCs to treat different musculoskeletal pathologies. The aim of this article is to describe the protocol for the treatment of osteochondral lesions of the talus using microfractured and purified adipose tissue in association with arthroscopic microperforations. The protocol involves several critical steps with high risks of complications. During fat harvesting, complications can be divided into local or systemic complications....

Arthroscopy is the gold standard for the treatment of osteochondral lesions of the talus (OLTs) with the aim of pain relief, restoring functionality, and improving quality of life, especially in young and active patients.
Currently, arthroscopic techniques can be classified in three ways. The reparative technique stimulates cells derived from bone marrow through a debridement and microperforations at the level of lesion. The reconstructive technique replaces the lesion using an autologous or heterologous ostechondral graft. The regenerative technique exploits the ability of multipotent cells to differentiate and replicate to reconstruct the damaged tissue1,2,3,4,5,6.
In recent years , regenerative techniques have been the subject of numerous in vitro and in vivo studies for the treatment of OLTs, and particularly mesenchymal stem cells derived from adipose tissue (ADSCs)7,8,9. These mesenchymal stem cells exhibit morphological and functional characteristics similar to other multipotent cells, isolated from other tissues; they also have the ability to differentiate into several and different cellular lines both in vitro and in vivo10,11,12,13. The focus on research regarding these cells is mainly due to their localization, in fact they represent from 10% to 30% of normal body weight with a concentration of 5,000 cells per gram of tissue13,14. On the other hand, a factor that limits the use of these cells is related to their handling during laboratory procedures. The lipoaspirate containing aggregates of adipocytes, collagen fibers, and normal vascular components is enzymatically processed with collagen A type I, and subjected to hemolysis before culture. The aim here is to describe the protocol for the treatment of osteochondral lesions of the talus using microfractured and purified adipose tissue.

<table><tbody><tr><td>PROCESS KIT - PROCESSING KIT FOR FAT TISSUE</td><td>LIPOGEMS</td><td>LG PK 60</td><td>Lipogems Kit to obtain microfractured and purified ADSCs</td></tr><tr><td>HINTERMANN SPREADER</td><td>INTEGRA</td><td>119654</td><td>The spreader allow to access most of the talar dome, in particular in case of posterior lesion</td></tr><tr><td>CUP CURETTE</td><td>ARTHREX</td><td>AR-8655-02</td><td>To remove the damaged cartilage and necrotic and sclerotic bone</td></tr><tr><td>CHONDRAL PICK 30&amp;deg; TIP / 60&amp;deg; TIP</td><td>ARTHREX</td><td>AR-8655-05&lt;br/&gt; AR-8655-06</td><td>To perfrom microperforation at the level of the lesion, stimulating bleeding from the subchondral bone</td></tr><tr><td>SHAVER</td><td>ARTHREX</td><td>AR-7300SR</td><td>To clean the joint and aspirate water</td></tr></tbody></table>

autologous microfractured and purified adipose tissue for arthroscopic management of osteochondral lesions of the talus

Autologous Microfractured and Purified Adipose Tissue for Arthroscopic Management of Osteochondral Lesions of the Talus

In recent years, regenerative techniques have been increasingly studied and used to treat osteochondral lesions of the talus. In particular, several studies have focused their attention on mesenchymal stem cells derived from adipose tissue. Adipose-derived stem cells (ADSCs) exhibit morphological characteristics and properties similar to other mesenchymal cells, and are able to differentiate into several cellular lines. Moreover, these cells are also widely available in the subcutaneous tissue, representing 10 - 30% of the normal body weight, with a concentration of 5,000 cells per gram of tissue.
In the presented technique, the first step involves harvesting ADSCs from the abdomen and a process of microfracture and purification; next, the surgical procedure is performed entirely arthroscopically, with less soft tissue dissection, better joint visualization, and a faster recovery compared with standard open procedures. Arthroscopy is characterized by a first phase in which the lesion is identified, isolated, and prepared with microperforations; the second step, performed dry, involves injection of adipose tissue at the level of the lesion.
Between January 2016 and September 2016, four patients underwent arthroscopic treatment of osteochondral lesion of the talus with microfractured and purified adipose tissue. All patients reported clinical improvement six months after surgery with no reported complications. Functional scores at the latest follow-up are encouraging and confirm that the technique provides reliable pain relief and improvements in patients with osteochondral lesion of the talus.

Between January 2016 and September 2016, four patients underwent arthroscopic treatment of osteochondral lesion of the talus with microfractured and purified adipose tissue. All patients reported clinical improvement six months after surgery. Preliminary clinical results are reported in Table 1. No complications were reported.
In recent years, the use of ADSCs for the treatment of foot and ankle pathologies has ...

The aim of this study is to report a protocol for the arthroscopic treatment of osteochondral lesions of the talus using microfractured and purified adipose-derived stem cells.

In recent years, regenerative techniques have been increasingly studied and used to treat osteochondral lesions of the talus. In particular, several ...

autologous-microfractured-purified-adipose-tissue-for-arthroscopic

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This protocol describes the isolation of adipose-derived stromal cells from lipoaspirate and the creation of a 4 mm critical-sized calvarial defect to evaluate skeletal regeneration.

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

An experimental technique for the treatment of chondral defects in the rabbit's knee joint is described. The implantation of autologous chondrocytes seeded on a matrix is a well-accepted method for the remodeling and repair of articular cartilage lesions providing satisfying long-term results. Matrix-assisted autologous chondrocyte transplantation (MACT) offers a standardized and clinically established implantation method.

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

An experimental technique for the treatment of osteochondral defects in the rabbit's knee joint is described. The implantation of allogeneic mesenchymal stem cells into osteochondral defects provides a promising development in the field of tissue engineering. The preparation of fibrin-cell-clots in vitro offers a standardized method for implantation.

Treatment of Osteochondral Defects in the Rabbit's Knee Joint by Implantation of Allogeneic Mesenchymal Stem Cells in Fibrin Clots

We present our preoperative, operative, and postoperative protocols for the treatment of osteoarthritis of the ankle with total ankle replacement via lateral transfibular approach.

Treatment of Ankle Osteoarthritis with Total Ankle Replacement Through a Lateral Transfibular Approach

Here, we present a protocol to produce an adipose tissue-derived stromal vascular fraction and its application to improve knee functions by regenerating cartilage-like tissue in human patients with osteoarthritis.

Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration

Here, we present human adipose tissue enzyme-free micro-fragmentation using a closed system device. This new method allows the obtainment of sub-millimeter clusters of adipose tissue suitable for in vivo transplantation, in vitro culture, and further cell isolation and characterization.

Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

Primary tissues obtained from patients following total knee arthroplasty provide an experimental model for osteoarthritis research with maximal clinical translatability. This protocol describes how to identify, process, and isolate RNA from seven unique knee tissues to support mechanistic investigation in human osteoarthritis.

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

This protocol details the isolation of chondrocytes, Fibronectin Adhesion Assay-derived Chondroprogenitors (FAA-CPs), and Migratory Chondroprogenitors (MCPs) from human articular cartilage. It covers enzymatic digestion, fibronectin adhesion, and migration-based assays for isolating and characterizing these cells.

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

The proposed protocol entails a global approach to assess bone formation in the context of bone regeneration using multimodal analyses. It aims to provide qualitative and quantitative information on new bone formation, enhancing the rigor and validity of basic and pre-clinical investigations.

Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model

Here, we present an integrated protocol for generating controlled osteochondral lesions in sheep knees to establish an experimental model suitable for bone and cartilage tissue regeneration studies. The protocol outlines a method for inducing a full-thickness osteochondral defect in the femoral condyle, utilizing techniques that minimize animal welfare impacts and variability in results.

Establishment and Evaluation of a Sheep Model of Full-thickness Osteochondral Defect

Learn arthroscopic treatment of osteochondral talar lesions using autologous microfractured adipose-derived stem cells. This technique enables cartilage regeneration with minimal soft tissue dissection and faster patient recovery.

Autologous Microfractured and Purified Adipose Tissue for Arthroscopic Management of Osteochondral Lesions of the Talus

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