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Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

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Cancer Research

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JoVE Journal Cancer Research

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

DOI:

10.3791/56418-v

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09:09 min

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November 10, 2017

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Magdalena Delgado*¹, Anisha Kothari*¹, Walter N. Hittelman, Timothy C. Chambers

¹Department of Biochemistry and Molecular Biology,University of Arkansas for Medical Sciences, ²Department of Experimental Therapeutics,University of Texas MD Anderson Cancer Center

Chapters

00:05Title
00:41Assembling the Elutriation System
02:55Priming the Elutriation System
04:56Introduction of Cell Sample into the Elutriation System and Collection of Fractions
06:56Results: Evaluation of Centrifugal Elutriation-based Sorting of Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases
08:21Conclusion

Summary

Automatic Translation

This protocol describes the use of centrifugal elutriation to separate primary acute lymphoblastic leukemia cells into different cell cycle phases.

Tags

Keywords: Primary Acute Lymphoblastic Leukemia Cell Cycle Phases Centrifugal Elutriation Cell Separation Cancer Therapeutics Anti-cancer Compounds Cell Isolation Physical Properties Cell Cycle Strobe Assembly Rotor Elutriation Chamber Rotating Seal Assembly Flow System Buffer Variable Speed Pump Sterilization 5% Bleach

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