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非遗传表面修饰的素-生物素系统在牛分枝杆菌卡介苗中的外源抗原表达
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Immunology and Infection
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JoVE Journal Immunology and Infection
Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System
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非遗传表面修饰的素-生物素系统在牛分枝杆菌卡介苗中的外源抗原表达

DOI:
10.3791/56421-v

•

10:11 min

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January 31, 2018

•

, , , ,
1Division of Infectious Diseases, Department of Medicine and Vancouver Costal Health Research Institute,University of British Columbia, 2Institute of Biomedical Technology,University of Tampere

Chapters

  • 00:05Title
  • 01:00Monomeric Avidin Fusion Protein Expression, Purification, and Refolding
  • 03:53Biotinylation of BCG Cell Surface
  • 05:47Binding of Monomeric Adivin-fusion Protein to Biotinylated BCG Surface
  • 07:06Results: Biotinylation with Avidin-OVA
  • 09:13Conclusion

Summary

Automatic Translation

Automatic Translation

提出了一种新的细菌表面快速抗原显示技术, 它涉及表面法, 其次是暴露于与单体素融合的感兴趣的蛋白质。用选定抗原载入卡介苗, 成功地提高了其免疫原性, 表明表面修饰可以取代传统的遗传方法。

Tags

Mycobacterium Bovis BCGSurface DecorationAvidin-biotin SystemExogenous Antigen ExpressionRecombinant ProteinE. Coli BL21Nickel NTA ColumnProtein RefoldingProtein Purification

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