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Journal / Immunology and Infection / Udtryk af eksogene antigener i Mycobacterium…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Udtryk af eksogene antigener i Mycobacterium bovis BCG Vaccine via ikke-genetiske overflade dekoration med begærlighed-biotin System
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Udtryk af eksogene antigener i Mycobacterium bovis BCG Vaccine via ikke-genetiske overflade dekoration med begærlighed-biotin System

Article DOI: 10.3791/56421-v • 10:11 min • January 31st, 2018
January 31st, 2018 •
1, 1, 1, 2, 1
1Division of Infectious Diseases, Department of Medicine and Vancouver Costal Health Research Institute, University of British Columbia, 2Institute of Biomedical Technology, University of Tampere

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En ny teknik til hurtig antigen visning på en bakteriel overflade præsenteres, som indebærer overflade biotinylation efterfulgt af udsættelse for proteiner af interesse i fusion med monomere begærlighed. Lastning BCG med udvalgte antigener med held forbedrer sin immunogenicitet, tyder på, at overflade dekoration kan erstatte traditionelle genetiske metoder.

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Immunologi sag 131 Mycobacterium tuberculosis makrofager immunrespons rekombinante proteiner T-celler antigener Biotin begærlighed-biotin
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