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Journal / Immunology and Infection / Uttrykk for eksogene antigener i Mycobacterium…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Uttrykk for eksogene antigener i Mycobacterium bovis BCG vaksinen via ikke-genetiske overflaten dekorasjon med Avidin-biotin System
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Uttrykk for eksogene antigener i Mycobacterium bovis BCG vaksinen via ikke-genetiske overflaten dekorasjon med Avidin-biotin System

Article DOI: 10.3791/56421-v • 10:11 min • January 31st, 2018
January 31st, 2018 •
1, 1, 1, 2, 1
1Division of Infectious Diseases, Department of Medicine and Vancouver Costal Health Research Institute, University of British Columbia, 2Institute of Biomedical Technology, University of Tampere

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En ny teknikk for rask antigen visning på en bakteriell overflate presenteres, som innebærer overflate biotinylation etterfulgt av eksponering for proteiner interesse sammensmelting med monomerisk avidin. Lasting BCG valgte antigener vellykket forbedrer sin immunogenisitet, antyder at landtransport dekorasjon kan erstatte tradisjonelle genetisk tilnærminger.

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Immunologi problemet 131 Mycobacterium tuberculosis makrofager immunforsvaret rekombinante proteiner T-celler antigener Biotin avidin-biotin
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