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Journal / Immunology and Infection / La surexpression et la purification de l'être …
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Immunology and Infection

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JoVE Journal Immunology and Infection
La surexpression et la purification de l'être humain
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La surexpression et la purification de l'être humain

Article DOI: 10.3791/56430-v • 07:35 min • August 3rd, 2017
August 3rd, 2017 •
*1, *1, 2, 2, 1,3, 1,3, 2, 2,4, 2
1Sackler Faculty of Medicine, Tel Aviv University, 2Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 3Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University, 4Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University
* These authors contributed equally

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Un protocole simple pour la surexpression et la purification de la cis- prényltransférase humaine optimisée par codon, dans des conditions non dénaturantes, d' Escherichia Coli , est décrit, avec un dosage d'activité enzymatique. Ce protocole peut être généralisé pour la production d'autres protéines de cis- prenyltransférase en quantité et en qualité adaptées aux études mécanistiques.

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Immunologie Numéro 126 optimisation de codons surexpression hétérologue purification de protéines prényltransférase déshydrodolichyl diphosphate synthase polyprenyl
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