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Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System
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Neuroscience
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JoVE Journal Neuroscience
Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

DOI:
10.3791/56436-v

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09:52 min

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December 28, 2017

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, , , , , , , ,
1Department of Biochemistry and Molecular Biology and Molecular, Cellular and Integrated Neuroscience Program,Colorado State University, 2IBMC-Instituto de Biologia Molecular e Celular, i3S-Instituto de Investigaçãoe Inovação em Saúde, ICBAS,Universidade do Porto, 3Denali Therapeutics

Chapters

  • 00:05Title
  • 01:28Preparation of Roller Tubes and Coverslips
  • 02:51Plating Slices
  • 05:44Slice Imaging
  • 07:28Results: Repetitive Intermittent Imaging of Brain Slices During Long-term Culture
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a modified roller tube method for culturing and intermittent high-resolution imaging of rodent brain slices over many weeks with precise repositioning on photoetched coverslips. Neuronal viability and slice morphology are well maintained. Applications of this fully enclosed system using viruses for cell-type specific expression are provided.

Tags

Keywords: Brain Slice CultureLong-term CultureRepetitive ImagingFluorescence ImagingEnclosed SystemRoller Tube MethodPhoto Etched CoverslipsViral VectorsNeurological DisordersSynapse AlterationRodent Models

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