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JoVE Journal Developmental Biology
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Un base di RANKL Osteoclast cultura Assay di midollo osseo del topo per studiare il ruolo di mTORC1 nella formazione degli osteoclasti

Article DOI: 10.3791/56468-v • 09:37 min • March 15th, 2018
March 15th, 2018 •
*1, *2, *1, 3, 2, 1, 2, 1
1Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine
* These authors contributed equally

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Questo manoscritto descrive un protocollo a isolare e cultura gli osteoclasti in vitro da midollo osseo di topo e di studiare il ruolo della destinazione dei mammiferi/meccanicistica della rapamicina complesso 1 nella formazione degli osteoclasti.

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Biologia dello sviluppo problema 133 Osteoclast mTORC1 Raptor ossea rimodellamento biologia cellulare RANKL M-CSF
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