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ミトコンドリア由来ペプチド治療時に ERK の活性化のための細胞レベル下の分別
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JoVE Journal
Biology
Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment
Please note that all translations are automatically generated.
Click here for the English version.
ミトコンドリア由来ペプチド治療時に ERK の活性化のための細胞レベル下の分別
DOI:
10.3791/56496-v
•
07:55 min
•
September 25, 2017
•
Su-Jeong Kim
,
Jialin Xiao
,
Pinchas Cohen
,
Kelvin Yen
1
Leonard Davis School of Gerontology
,
University of Southern California
Chapters
00:05
Title
00:52
Peptide Treatment to Cells
01:46
Subcellular Fractionation of Cytosol and Nucleus
03:31
Isolate Crude Mitochondrial Fraction
05:43
Results: Analysis of ERK Activation Upon Mitochondrial-derived Peptide Treatment
07:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルでは、ミトコンドリア由来ペプチドと細胞を刺激し、シグナル伝達カスケードとリン蛋白質の局在化を評価する方法について説明します。
Tags
Subcellular Fractionation
ERK Activation
Mitochondrial-derived Peptide
Signaling Cascade
Humanin
SH-SY5Y Cells
HEK293 Cells
Cytoplasmic Fraction
Nuclear Fraction
Cytosol Fraction
Nuclear Extraction Buffer
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