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An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
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Immunology and Infection
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JoVE Journal Immunology and Infection
An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

DOI:
10.3791/56515-v

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09:43 min

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March 30, 2018

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, , , , , ,
1Genetic Resources Center, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, 2MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, 3State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences

Chapters

  • 00:04Title
  • 01:35Isolation of PBMC from CAEBV Patients
  • 02:59Expansion of Cells and Numbering the Viable Cells
  • 04:08Preparation for Cell Phenotyping by Flow Cytometry
  • 05:52Expansion and Cryopreservation of NK/T Cells
  • 07:23Results: Representative Gating Strategy for Flow Cytometry Analysis of Cell Lines L196 and M296
  • 08:25Conclusion

Summary

Automatic Translation

Automatic Translation

A simple method for obtaining NK and T cell clones from CAEBV patients was developed with high efficiency, a small amount of peripheral blood, and a low-dose of IL-2.

Tags

Keywords: NK CellsT CellsEpstein-Barr VirusCAEBVCell Line EstablishmentCell CulturePBMCIL-2Cell ProliferationCell Viability

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