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Journal / Immunology and Infection / 饱和脂肪酸诱导神经酰胺相关巨噬细胞死亡…
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Immunology and Infection

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JoVE Journal Immunology and Infection
饱和脂肪酸诱导神经酰胺相关巨噬细胞死亡
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饱和脂肪酸诱导神经酰胺相关巨噬细胞死亡

Article DOI: 10.3791/56535-v • 08:26 min • October 31st, 2017
October 31st, 2017 •
1, 1, 1, 1
1Department of Microbiology and Immunology, University of Louisville

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本文阐述了一种从骨髓细胞中提取小鼠原发巨噬蛋白的方法, 并提出了制备 BSA-脂肪酸共轭物的简便方法。然后我们证明饱和脂肪酸能诱导巨噬细胞死亡, 这种细胞死亡与神经酰胺水平的细胞积累呈正相关。

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免疫学 问题 128 骨髓来源的巨噬细胞 BSA (牛血清白蛋白) 饱和脂肪酸 棕榈酸 硬脂酸 神经酰胺 细胞死亡
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